Program namemaster degree in Applied Biochemistry

Natural and life Sciences Departement

Title of the Master: Applied Biochemistry

Semester : S1
EU title: Fundamental
Title of the subject: Molecular and cellular biology I
Teaching objectives (Describe what the student is supposed to have acquired as skills after success at this
material – maximum 3 lines).
Know the molecular and cellular bases necessary to exploit the experimental results of the interaction of
molecules with ligands in biological systems
Recommended previous knowledge (brief description of the knowledge required to be able to follow this
teaching – Maximum 2 lines). Biochemistry, genetics
Content of the subject (it is mandatory to indicate the detailed content of the Program and personal work)
Program

• Courses

1. The cell cycle

• The cell cycle
• The regulation of the cell cycle
• The transition from G2 to M: the triggering of mitosis
• Mechanisms for monitoring G1/S, G2/M and Metaphase/Anaphase transitions
• Apoptosis
• Cancer

1. Genetic diseases and gene therapy

• Genetic diseases
• Gene therapy

1. Modes of protein transport: translocation, diffusion, vesicle-tubular transport
   Compartments of eukaryotic cells (Cell Organelles) Intracellular trafficking of proteins

• TD
• TD1: The surveillance mechanisms controlling the cell cycle
• TD2: The apoptotic pathways: extrinsic and intrinsic
• TD3: Therapeutic strategies against cancer
• TD4: Addressing proteins
• Others (Personal work)
  -Preparation of TD series
  -Bibliographic research.
  Semester : 1
  EU title: Fundamental
  Title of the subject: Study of macromolecule-ligand interactions
  Teaching objectives (Describe what the student is supposed to have acquired as skills after success at this
  material – maximum 3 lines).
  Study of the different interactions existing between proteins and ligands.
  Recommended previous knowledge (brief description of the knowledge required to be able to follow this
  teaching – Maximum 2 lines). Biochemistry
  Content of the subject (it is mandatory to indicate the detailed content of the face-to-face program and personal work)
  Program
• Courses

1. Mechanisms of folding of soluble proteins
2. Interactions proteins ligands
   General Information

• Concept of thermodynamics
• Nature of the ligands
• Nature of the interactions
  Methods for studying protein-ligand interactions
• Spectroscopic methods (FRET case, BRET, …etc) Optical methods (RSP case, …etc).
• Calorimetric methods (ITC,……etc).
  Analysis of the protein-ligand interaction
• Case of a single site
• Case of several sites
  Examples of protein-ligand interaction
  Protein-Nucleic Acid Enzyme-Receptor Substrate-Hormone
  *TD
• TD1: The folding of proteins.
• TD2: Protein-ligand interaction
• Others (Personal work)
  -Bibliographic research.
  -Preparations for the TD series.
  Semester : 1
  EU title: Fundamental
  Title of the material: Structure and function of macromolecules
  Teaching objectives (Describe what the student is supposed to have acquired as skills after success at this
  material – maximum 3 lines).
  Acquire in-depth knowledge on the chemical determination of the structures of biopolymers and their syntheses
  chemical properties as well as the three-dimensional structure of proteins.
  Recommended previous knowledge (brief description of the knowledge required to be able to follow this
  teaching – Maximum 2 lines).Biochemistry
  Content of the subject (it is mandatory to indicate the detailed content of the Program and personal work)
  Program
• Courses

1. Structure and function of globular proteins Reminder about proteins and
   amino acids. Globular protein structure.
   -Notion of motive.
   -Concept of domain
   -Case example: Myoglobin and hemoglobin
2. Structure and function of membrane proteins Methods of protein studies
   extraction and purification of membrane proteins Extraction and